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- DNA mismatch repair is a system for recognizing and repairing erroneous insertion, deletion and mis-incorporation of bases that can arise during DNA replication and recombination, as well as repairing some forms of DNA damage. Mismatch repair is strand-specific. During DNA synthesis the newly synthesised strand will include errors commonly. In order to do this the mismatch repair machinery distinguishes the newly synthesised strand from the template. In gram-negative bacteria transient hemimethylation distinguishes the strands. In other prokaryotes and eukaryotes the exact mechanism is not clear. It is suspected that in eukaryotes, newly synthesized lagging-strand DNA transiently contains nicks and provides a signal that directs mismatch proofreading systems to the appropriate strand. This implies that these nicks must be present in the leading strand, and evidence for this has recently been found. Recent work has shown that nicks are sites for RFC-dependent loading of the replication sliding clamp PCNA, in an orientation-specific manner, such that one face of the donut shaped protein is juxtaposed towards the 3'-OH end at the nick. Oriented PCNA then directs the action of the MutLalpha endonuclease to one strand in the presence of a mismatch and MutSalpha or MutSbeta.
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"dna mismatch repair." Anagrams.net. STANDS4 LLC, 2024. Web. 13 May 2024. <https://www.anagrams.net/dna%20mismatch%20repair>.
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