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The Assassin's Creed media franchise, which primarily consists of a series of open-world action-adventure stealth video games published by Ubisoft, features an extensive cast of characters in its historical fiction and science fiction-based narratives. The series also encompasses a wide variety of media outside of video games, including novels, comic books, board games, animated films, a live-action film, and an upcoming Netflix television series. The series features original characters intertwined with real-world historical events and figures, and is centered on a fictional millennia-old struggle for peace between the Assassin Brotherhood, inspired by the real-life Order of Assassins, who fight for peace and free will and embody the concept of chaos; and the Templar Order, inspired by the real-life Knights Templar, who desire peace through control over all of humanity, and embody the concept of order. A convention established by the first game involves the player experiencing the lives of these characters as part of a simulation played by a protagonist from the modern day, using technology known as the Animus developed by Abstergo Industries, a corporate front of the Templar Order in the modern era. The first five games feature modern-day protagonist Desmond Miles, a direct descendant of their respective lead characters who are members of familial lines that had sworn an allegiance to the Assassins. By exploring his ancestors' memories, Desmond searches for powerful artifacts called "Pieces of Eden", which are connected to the Isu, a precursor race that created humanity to serve them and went extinct following a catastrophic event tens of thousands of years ago. However, they left behind clues to guide humanity to their technology, which could be used to prevent the same disaster from happening in the future. Following the events of Assassin's Creed III, Abstergo develops a more advanced version of the Animus technology called the Helix, which can explore the genetic memories of any historical individual using their DNA without relying on the user being a direct descendant of them. From Assassin's Creed IV: Black Flag to Assassin's Creed Syndicate, the player assumes control of unnamed research analysts working for the entertainment branch of Abstergo or the Assassin Brotherhood; the analysts are intended to be the embodiment of the player in the Assassin's Creed universe. From Assassin's Creed Origins to Assassin's Creed Valhalla, the modern-day protagonist is Layla Hassan, an ambitious former Abstergo employee who developed a portable version of Animus technology and is eventually recruited to the Brotherhood. This article describes major historical and fictional characters that appear in the video games and the 2016 live-action film adaptation. Most games tend to feature standalone or self-contained stories told within a fictionalized version of real-world historical civilizations, with at least one lead character from that setting and time period. However, some games are more interconnected than others, as is the case with the "Ezio Trilogy", consisting of Assassin's Creed II, Brotherhood, and Revelations. These games feature interconnected characters and plot points, so to avoid listing a character multiple times, this article only takes into account their first or most significant appearance and describes their entire history there.

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